Eligibility Details:
Inclusion Criteria: men and pre-menopausal women Body mass index: 20-35 kg/m2 Body weight >
than 50 kg Self-reported stable body weight during the prior six months
Exclusion Criteria:
Fasting glucose >125 mg/dl Evidence of liver disorder (AST or ALT >200% upper limit of
normal range) Evidence of kidney disorder (>2.0 mg/dl creatinine) Evidence of thyroid
disorder (out of normal range) Systolic blood pressure consistently over 140 mmHg or
diastolic blood pressure over 90 mmHg Triglycerides > 400 mg/dl LDL-C > 160 mg/dl in
combination with Chol:HDL > 4 Hemoglobin < 10 g/dL Pregnant or lactating women Current,
prior (within 12 months), or anticipated use of any hypolipidemic or anti-diabetic agents.
Use of thyroid, anti-hypertensive, anti-depressant, weight loss medications or any other
medication which, in the opinion of the investigator, may confound study results Use of
tobacco Strenuous exerciser (>3.5 hours/week at a level more vigorous than walking) Surgery
for weight loss Diet exclusions: Food allergies, special dietary restrictions, routine
consumption of less than 3 meals/day, routine ingestion of more than 2 sugar-sweetened
beverages or 1 alcoholic beverage/day, unwillingness to consume any food on study menu
Veins that are assessed by the R.N.s as being unsuitable for long-term infusions and
multiple blood draws from a catheter.
Pre-existing claustrophobia or metal implants that preclude magnetic resonance imaging Any
other condition that, in the opinion of the investigators, would put the subject at risk
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