“Cold Apheresis Platelets in PAS”
This is a platelet transfusion study. The purpose of this study is to measure the life span and quality of platelets stored in a refrigerator. Participants will give platelets by apheresis. Platelets will be stored for 3 -20 days. A small portion of the subject's own stored platelets will be tagged with a radioactive isotope and infused back into the participant. This will enable us to track how many transfused platelets survive after storage in the refrigerator.
Procedure - Apheresis platelet collection
A single apheresis platelet unit will be collected from a healthy adult volunteer subject using the Trima AccelÂ® Automated Blood Collection System. Concurrent plasma will be collected.
Refrigerated storage of apheresis platelets in PAS/plasma
The apheresis platelet unit will be stored, without agitation, at 4Â°C, for up to 20 days.
Radiation - Infusion of aliquot of autologous radiolabeled platelets
Fresh and stored aliquots will be labeled with Indium-111. We are administering â‰¤30 microcuries in both infusions for a total dose of 0.8 mSv.
In vitro tests
Tests to evaluate platelet function will be performed on the platelet unit on day of collection and end of storage
Cold Apheresis Platelets in PAS