Transfusion of Biotin-Labeled Red Blood Cells

Eligibility Details: Inclusion Criteria: - Ages 18 years and older - Weight ≥110 lbs - Hemoglobin ≥ 12.5 g/dL or hematocrit ≥ 38% for women and hemoglobin ≥ 13.0 g/dL or hematocrit ≥ 39% for men. - Meet criteria for autologous blood donation Exclusion Criteria: - Subjects with a past medical history or symptoms of blood dyscrasia, diabetes mellitus, hyperlipidemia, obstructive sleep apnea, renal disease, congestive heart failure, significant cardiac disease and / or known peripheral arterial disease. - Moderate to severe systemic hypertension (systolic blood pressure >140 mmHg and/or diastolic blood pressure > 95 mmHg - Systolic blood pressure <100 mmHg and/or diastolic blood pressure < 60 mmHg on the study day. - Positive Direct Antiglobulin Test - Consumption of biotin supplements or raw eggs within 30 days - Treatment with antibiotics in the week before initiating study participation to avoid suppression of erythropoiesis, which may accompany infection. - Blood loss in the previous 8 weeks due to epistaxis, trauma, hemoptysis, gastrointestinal bleeding, diagnostic phlebotomy (> 30 ml) - Subjects who report tobacco or marijuana smoking within 6 months of study. - Cognitively impaired subjects, or institutionalized persons and subjects unable or unwilling to complete written informed consent - Subjects with a history of blood donation within the last 56 days. - Use of other investigational drugs/devices within 30 days of screening. - Subjects taking any medication for the treatment of diabetes including insulin - Females of childbearing potential who are pregnant or unwilling to undergo pregnancy testing; females with positive pregnancy testing on screening day will be excluded. - History of prior transfusion reaction to blood products. - Allergic reaction to biotin - Donors with naturally occurring antibodies against BioRBCs will be excluded from the study